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Development of a Bolometer Detector System for the NIST High Accuracy Infrared Spectrophotometer.

Development of a Bolometer Detector System for the NIST High Accuracy Infrared Spectrophotometer.

Posted on July 23, 2021July 23, 2021 by Patricia

A bolometer detector system was developed for the excessive accuracy infrared spectrophotometer at the National Institute of Standards and Technology to supply most sensitivity, spatial uniformity, and linearity of response masking the whole infrared spectral vary. The spatial response variation was measured to be inside 0.1 %.

The linearity of the detector output was measured over three many years of enter energy. After making use of a easy correction process, the detector output was discovered to deviate lower than 0.2 % from linear habits over this vary. The noise equal energy (NEP) of the bolometer system was 6 × 10-12 [Formula: see text] at the frequency of 80 Hz.

The detector output three dB roll-off frequency was 200 Hz. The detector output was steady to inside ± 0.05 % over a 15 min interval. These outcomes show that the bolometer detector system will function a superb detector for the excessive accuracy infrared spectrophotometer.

Our outcomes do nevertheless point out kinetic variations between actinic gentle sources (laser vs. LED), and we’ve tried to resolve these results by various our LED gentle depth and period. The standardized configuration of this spectrophotometer will even enable a extra uniform kinetic evaluation of samples in several laboratories.

We can conclude that our findings from the LED-based system show an added complete protein focus impact because of a number of turnover occasions of P700+ discount by cyt c 6 throughout the longer illumination regime.

Instrumentation-related uncertainty of reflectance and transmittance measurements with a two-channel spectrophotometer.

Spectrophotometers are operated in quite a few fields of science and trade for a selection of purposes. In order to supply confidence for the measured knowledge, analyzing the related uncertainty is effective. However, the uncertainty of the measurement outcomes is usually unknown or decreased to sample-related contributions.

In this paper, we describe our strategy for the systematic dedication of the measurement uncertainty of the commercially accessible two-channel spectrophotometer Agilent Cary 5000 in accordance with the Guide to the expression of uncertainty in measurements.

We concentrate on the instrumentation-related uncertainty contributions relatively than the particular utility and thus define a normal process which will be tailored for different devices. Moreover, we uncover a systematic sign deviation because of the inertia of the measurement amplifier and develop and apply a correction process. Thereby we enhance the usable dynamic vary of the instrument by a couple of order of magnitude.

We current strategies for the quantification of the uncertainty contributions and mix them into an uncertainty finances for the system. A brand new reflectometer-spectrophotometer has been designed and constructed utilizing state-of-the-art expertise to boost optical properties of supplies measurements over the ultraviolet, seen, and near-infrared (UV-Vis-NIR) wavelength vary (200 nm to 2500 nm).

The instrument, Spectral Tri-function Automated Reference Reflectometer (STARR), is succesful of measuring specular and diffuse reflectance, bidirectional reflectance distribution operate (BRDF) of diffuse samples, and each diffuse and non-diffuse transmittance. Samples as much as 30 cm by 30 cm will be measured. The instrument and its characterization are described.

Deconvolution of ferredoxin, plastocyanin, and P700 transmittance modifications in intact leaves with a new kind of kinetic LED array spectrophotometer.

A newly developed compact measuring system for evaluation of transmittance modifications in the near-infrared spectral area is described; it permits deconvolution of redox modifications because of ferredoxin (Fd), P700, and plastocyanin (PC) in intact leaves. In addition, it could additionally concurrently measure chlorophyll fluorescence.
The main opto-electronic elements in addition to the ideas of knowledge acquisition and sign deconvolution are outlined. Four authentic pulse-modulated dual-wavelength distinction alerts are measured (785-840 nm, 810-870 nm, 870-970 nm, and 795-970 nm).
Deconvolution is predicated on particular spectral info introduced graphically in the type of ‘Differential Model Plots’ (DMP) of Fd, P700, and PC which are derived empirically from selective modifications of these three elements underneath appropriately chosen physiological situations.
Development of a Bolometer Detector System for the NIST High Accuracy Infrared Spectrophotometer.
Whereas info on maximal modifications of Fd is obtained upon illumination after dark-acclimation, maximal modifications of P700 and PC will be readily induced by saturating gentle pulses in the presence of far-red gentle. Using the info of DMP and maximal modifications, the new measuring system allows on-line deconvolution of Fd, P700, and PC.
The efficiency of the new system is demonstrated by some examples of sensible purposes, together with quick measurements of flash rest kinetics and of the Fd, P700, and PC modifications paralleling the polyphasic fluorescence rise upon utility of a 300-ms pulse of saturating gentle.

Calibration Transfer Between a Bench Scanning and a Submersible Diode Array Spectrophotometer for In Situ Wastewater Quality Monitoring in Sewer Systems.

Online monitoring applications primarily based on spectroscopy have a excessive utility potential for the detection of hazardous wastewater discharges in sewer programs. Wastewater hydraulics poses a problem for in situ spectroscopy, particularly when the system contains storm water connections resulting in fast modifications in water depth, velocity, and in the water high quality matrix.

Thus, there’s a must optimize and repair the location of in situ devices, limiting their availability for calibration. In this context, the growth of calibration fashions on bench spectrophotometers to estimate wastewater high quality parameters from spectra acquired with in situ devices may very well be very helpful. However, spectra comprise info not solely from the samples, but in addition from the spectrophotometer typically invalidating this strategy.

The use of calibration switch strategies is a promising answer to this downside. In this research, calibration fashions had been developed utilizing interval partial least squares (iPLS), for the estimation of complete suspended solids (TSS) and chemical oxygen demand (COD) in sewage from Ultraviolet-visible spectra acquired in a bench scanning spectrophotometer.

The feasibility of calibration switch to a submersible, diode array tools, to be subsequently operated in situ, was assessed utilizing three procedures: slope and bias correction (SBC); single wavelength standardization (SWS) on imply spectra; and native centering (LC).

1000 ug/mL 2-Ethylhexyl diphenyl phosphate in HPLC Methanol

LCS-6041 Scientific Laboratory Supplies 1ML 285 EUR

1000 ug/mL 24-Diaminoanisole in HPLC Grade Methanol- 1mL

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1000 ug/mL Methomyl in HPLC Methanol Volume: 1mL Ampule

S-2395-MEOH Scientific Laboratory Supplies 1ML 129.96 EUR

Methanol

MC6700 Bio Basic 500ml 87.84 EUR

Methanol

MTH3800 ScyTek Laboratories 1 Gal. 220.8 EUR

Methanol

MTH500 ScyTek Laboratories 500 ml 140.4 EUR

Methanol

MTH999 ScyTek Laboratories 1000 ml 181.2 EUR

Methanol

GX5097-1L Glentham Life Sciences 1 l 55.2 EUR

Methanol

GX5097-500ML Glentham Life Sciences 500 ml 48 EUR

Methanol

S-2380 Scientific Laboratory Supplies 1ML 38.76 EUR

1000 ug/mL 3-Keto-Carbofuran in HPLC Methanol Volume: 1mL Ampule

S-6129 Scientific Laboratory Supplies 1ML 408.12 EUR

(Tributylstannyl)Methanol

20-abx180122 Abbexa
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Triphenyl methanol

20-abx184795 Abbexa
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(tributylstannyl)Methanol

20-abx184888 Abbexa
  • 13122.00 EUR
  • 552.00 EUR
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  • 100 g
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(4,4-Difluorocyclohexyl)methanol

20-abx181421 Abbexa
  • 944.40 EUR
  • 326.40 EUR
  • 594.00 EUR
  • 10 g
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(3,5-Dibromophenyl)methanol

20-abx182730 Abbexa
  • 243.60 EUR
  • 661.20 EUR
  • 343.20 EUR
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(2-Iodophenyl)methanol

20-abx184507 Abbexa
  • 376.80 EUR
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Azlon Wash Bottle 250ml for Methanol non-vented

BOT9358 Scientific Laboratory Supplies PK5 30.28 EUR

Azlon Wash Bottle 500ml for Methanol non-vented

BOT9374 Scientific Laboratory Supplies PK5 34.14 EUR

Benchmark Adapter Pack for Cryovials and HPLC Vials

CEN1871 Scientific Laboratory Supplies PK4 61.56 EUR

Pyrrolidin-2-Yl-Methanol

20-abx184343 Abbexa
  • 243.60 EUR
  • 526.80 EUR
  • 309.60 EUR
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2-Bromopyridine-4-methanol

20-abx185627 Abbexa
  • 226.80 EUR
  • 360.00 EUR
  • 292.80 EUR
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Methanol Assay Kit (Colorimetric)

K898-100 Biovision 796.8 EUR

500mL Wash Bottle Methanol

BOT0302 Scientific Laboratory Supplies PK5 36.71 EUR

Azlon Wash Bottle 250ml for Methanol with Driplock seal

BOT9338 Scientific Laboratory Supplies PK5 36.07 EUR

Azlon Wash Bottle 500ml for Methanol with Driplock seal

BOT9348 Scientific Laboratory Supplies PK5 43.32 EUR

Calib Std for Petrochem Methanol 3-ET-Toluene Toluene

REGASCAL-5-250 Scientific Laboratory Supplies 250ML 603.06 EUR

HPLC Mobile Phase 2

PCMP2 Scientific Laboratory Supplies 2.5L 169.86 EUR

HPLC Mobile Phase 3

PCMP3 Scientific Laboratory Supplies 2.5L 169.86 EUR

HPLC Mobile Phase 4

PCMP4 Scientific Laboratory Supplies 2.5L 128.82 EUR

HPLC Mobile Phase 5

PCMP5 Scientific Laboratory Supplies 2.5L 143.64 EUR

HPLC Mobile Phase 6

PCMP6 Scientific Laboratory Supplies 2.5L 134.52 EUR

(6-Methoxy-3-pyridyl)methanol

20-abx180107 Abbexa
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(1H-Pyrazol-4-yl)methanol

20-abx181387 Abbexa
  • 744.00 EUR
  • 1830.00 EUR
  • 1 g
  • 5 g

(2,6-Dichloro-3-pyridinyl)methanol

abx181396-1g Abbexa 1 g 410.4 EUR

(3-Bromothiophen-2-Yl)Methanol

20-abx181412 Abbexa
  • 1328.40 EUR
  • 410.40 EUR
  • 878.40 EUR
  • 10 g
  • 1 g
  • 5 g

(3-Chloropyrazin-2-Yl)Methanol

20-abx181414 Abbexa
  • 1245.60 EUR
  • 861.60 EUR
  • 1 g
  • 500 mg

(4-(tert-Butyl)phenyl)methanol

20-abx181420 Abbexa
  • 828.00 EUR
  • 326.40 EUR
  • 276.00 EUR
  • 100 g
  • 10 g
  • 5 g

(4-Benzylmorpholin-3-yl)methanol

20-abx181423 Abbexa
  • 510.00 EUR
  • 1128.00 EUR
  • 1 g
  • 5 g

(4-Bromo-2-pyridyl)methanol

20-abx181424 Abbexa
  • 427.20 EUR
  • 844.80 EUR
  • 1 g
  • 5 g

(5-Methoxypyridin-3-yl)methanol

20-abx181433 Abbexa
  • 1128.00 EUR
  • 510.00 EUR
  • 1 g
  • 250 mg

(5-Methylpyridin-3-Yl)Methanol

20-abx181434 Abbexa
  • 1028.40 EUR
  • 309.60 EUR
  • 610.80 EUR
  • 10 g
  • 1 g
  • 5 g

(S)-(Piperidin-3-yl)methanol

20-abx181463 Abbexa
  • 526.80 EUR
  • 1296.00 EUR
  • 1 g
  • 5 g

(2-Chloro-3-pyridinyl)methanol

20-abx182724 Abbexa
  • 811.20 EUR
  • 427.20 EUR
  • 276.00 EUR
  • 100 g
  • 25 g
  • 5 g

(3-Fluoropyridin-2-yl)methanol

abx182733-1g Abbexa 1 g 460.8 EUR

(2,5-dimethylfuran-3-yl)methanol

abx184841-1g Abbexa 1 g 644.4 EUR

(5-Fluoropyrimidin-2-yl)methanol

20-abx184858 Abbexa
  • 2749.20 EUR
  • 1228.80 EUR
  • 1 g
  • 250 mg

(5,6-Dimethoxypyridin-3-yl)methanol

abx186092-250mg Abbexa 250 mg 927.6 EUR

1000 ug/mLSabinene in Methanol

S-6645 Scientific Laboratory Supplies 1ML 129.96 EUR

D(+)-Trehalose dihydrate , ≥ 99% (HPLC)

T1036-010 GenDepot 100g 327.6 EUR

D(+)-Trehalose dihydrate , ≥ 99% (HPLC)

T1036-025 GenDepot 250g 565.2 EUR

GC/HPLC Demo Sample Pack

TNDPSSSV Scientific Laboratory Supplies EACH 3.77 EUR

HPLC Disposal Safety Can 7.5L

SAF7992 Scientific Laboratory Supplies EACH 535.8 EUR

Holder for Plasmid Midi, Maxi and Maxi plus, Ion Exchange column

FAPDE-holder-for-ion-exchange Favorgen 1 prep 189.6 EUR

Imidazo[1,2-A]Pyridine-8-Methanol

abx180784-1g Abbexa 1 g 861.6 EUR

(2-Amino-Pyridin-3-Yl)-Methanol

20-abx181398 Abbexa
  • 410.40 EUR
  • 610.80 EUR
  • 326.40 EUR
  • 10 g
  • 25 g
  • 5 g

(3-(Aminomethyl)oxetan-3-yl)methanol

20-abx181410 Abbexa
  • 861.60 EUR
  • 2380.80 EUR
  • 1 g
  • 5 g

(Tetrahydro-2H-Pyran-4-Yl)Methanol

20-abx182791 Abbexa
  • 610.80 EUR
  • 326.40 EUR
  • 100 g
  • 25 g

(5-(Benzyloxy)pyrimidin-2-yl)methanol

abx184855-1g Abbexa 1 g 1144.8 EUR

(Bicyclo[1.1.1]pent-1-yl)methanol

20-abx184865 Abbexa
  • 1830.00 EUR
  • 3567.60 EUR
  • 100 mg
  • 250 mg

(S)-(5,5-Dimethyltetrahydrofuran-2-yl)methanol

20-abx184878 Abbexa
  • 1846.80 EUR
  • 861.60 EUR
  • 1 g
  • 250 mg

N-Boc-1-Amino-Cyclobutyl-Methanol

abx188810-25g Abbexa 25 g 2614.8 EUR

1,6-Dioxaspiro[4.5]decan-2-methanol

TB00843 ChemNorm unit Ask for price

1000 ug/mL Prothioconazole in Methanol

LCS-4904 Scientific Laboratory Supplies 1ML 132.24 EUR

1000 ug/mL Toltrazuril in Methanol

LCS-5874 Scientific Laboratory Supplies 1ML 306.66 EUR

CP 0.1M Potassium Hydroxide in Methanol

CPMKOH01F Scientific Laboratory Supplies 1L 71.82 EUR

42 Component Terpene Kit in Methanol

CAN-TERP-KIT Scientific Laboratory Supplies 1ML 287.28 EUR

21 Component Terpene Mix in Methanol

CAN-TERP-MIX1 Scientific Laboratory Supplies 1ML 157.32 EUR

21 Component Terpene Mix in Methanol

CAN-TERP-MIX2 Scientific Laboratory Supplies 1ML 147.06 EUR

Azlon Wash Bottle 250ml Methanol Green

BOT9076 Scientific Laboratory Supplies PK5 22.95 EUR

Azlon Wash Bottle 500ml Methanol Green

BOT9090 Scientific Laboratory Supplies PK5 25.65 EUR

1000 ug/mL Sucrose in Methanol

S-3308 Scientific Laboratory Supplies 1ML 69.54 EUR

1000 ug/mL Fluorenone in Methanol

S-4332 Scientific Laboratory Supplies 1ML 88.92 EUR

1000 ug/mL Dimefuron in Methanol

S-4617 Scientific Laboratory Supplies 1ML 207.48 EUR

1000 ug/mL Cyanofenphos in Methanol

S-5958 Scientific Laboratory Supplies 1ML 188.1 EUR

1000 ug/mL lprovalicarb in Methanol

S-6029-MEOH Scientific Laboratory Supplies 1ML 124.26 EUR

1000 ug/mL Methoxyfenozide in Methanol

S-6033-MEOH Scientific Laboratory Supplies 1ML 74.1 EUR

1000 ug/mL Dimethylvinphos in Methanol

S-6042 Scientific Laboratory Supplies 1ML 174.42 EUR

1000 ug/mL Anilofos in Methanol

S-6100 Scientific Laboratory Supplies 1ML 180.12 EUR

1000 ug/mL Imibenconazole in Methanol

S-6107-MEOH Scientific Laboratory Supplies 1ML 308.94 EUR

Methanol, 50 % w/v, 100 ML

M-CSS-224 MiTeGen 4 x 25 ml 77 EUR
Description: Methanol, 50 % w/v, 100 ML

Omite 1000 ug.mL in HPLC Acetone

LCS-2895 Scientific Laboratory Supplies 1ML 148.2 EUR

1000 ug/mL Picoxystrobin HPLC Acetonitrile

LCS-5467 Scientific Laboratory Supplies 1ML 185.82 EUR

Kinesis upchurch HPLC Starter Fittings Kit

CHR2409 Scientific Laboratory Supplies EACH 267.91 EUR

Kinesis Shimadzu HPLC Preventative Maintenance Kit

CHR6259 Scientific Laboratory Supplies EACH 304.38 EUR

WHATMAN SPARTAN HPLC CERTIFIED SYRINGE F

WHA10463040 Scientific Laboratory Supplies PK100 335.16 EUR

WHATMAN SPARTAN HPLC CERTIFIED SYRINGE F

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WHATMAN SPARTAN HPLC CERTIFIED SYRINGE F

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WHATMAN SPARTAN HPLC CERTIFIED SYRINGE F

WHA10463100 Scientific Laboratory Supplies EACH 240.54 EUR

WHATMAN SPARTAN HPLC CERTIFIED SYRINGE F

WHA10463110 Scientific Laboratory Supplies PK100 273.6 EUR

1000ug/mL Lactose in HPLC Water

S-4516 Scientific Laboratory Supplies 1ML 77.52 EUR

(1-Methyl-1H-indol-6-yl)methanol

20-abx180082 Abbexa
  • 510.00 EUR
  • 2966.40 EUR
  • 1128.00 EUR
  • 1 g
  • 25 g
  • 5 g

(2-Phenyl-1H-imidazol-4-yl)methanol

20-abx180093 Abbexa
  • 543.60 EUR
  • 3266.40 EUR
  • 1228.80 EUR
  • 1 g
  • 25 g
  • 5 g

(R)-(-)-2,2-Dimethyl-1,3-dioxolane-4-methanol

20-abx181442 Abbexa
  • 276.00 EUR
  • 844.80 EUR
  • 410.40 EUR
  • 1 g
  • 25 g
  • 5 g

4-(hydroxymethyl)pyridin-3-yl]methanol hydrochloride

20-abx182009 Abbexa
  • 1579.20 EUR
  • 460.80 EUR
  • 1011.60 EUR
  • 10 g
  • 1 g
  • 5 g

[2-(4-Benzylpiperazin-1-yl)phenyl]methanol

20-abx182794 Abbexa
  • 477.60 EUR
  • 1028.40 EUR
  • 1 g
  • 5 g

[3-Chloro-5-(trifluoromethyl)-2-pyridinyl]methanol

20-abx182797 Abbexa
  • 1111.20 EUR
  • 878.40 EUR
  • 4236.00 EUR
  • 1 g
  • 500 mg
  • 5 g

1-Boc-4-Amino-Piperidine-4-Methanol

20-abx186138 Abbexa
  • 2715.60 EUR
  • 744.00 EUR
  • 410.40 EUR
  • 1897.20 EUR
  • 10 g
  • 1 g
  • 250 mg
  • 5 g

(1,3-Dimethyl-1H-pyrazol-5-yl)methanol

20-abx186706 Abbexa
  • 3032.40 EUR
  • 760.80 EUR
  • 326.40 EUR
  • 1296.00 EUR
  • 560.40 EUR
  • 100 g
  • 10 g
  • 1 g
  • 25 g
  • 5 g

1,5-Dimethyl-1H-pyrazol-3-yl-methanol

20-abx186708 Abbexa
  • 3951.60 EUR
  • 878.40 EUR
  • 326.40 EUR
  • 1562.40 EUR
  • 610.80 EUR
  • 100 g
  • 10 g
  • 1 g
  • 25 g
  • 5 g

(4-Amino-2-Methyl-5-Pyrimidinyl)Methanol

20-abx188004 Abbexa
  • 2097.60 EUR
  • 1045.20 EUR
  • 25 g
  • 5 g

(5-Bromo-2-Chloropyridin-4-Yl)Methanol

abx188016-25g Abbexa 25 g 1378.8 EUR

Bacillus methanolicus NAD-dependent methanol dehydrogenase (mdh)

1-CSB-EP330043BAU Cusabio
  • 733.20 EUR
  • 370.80 EUR
  • 2192.40 EUR
  • 1126.80 EUR
  • 1461.60 EUR
  • 476.40 EUR
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Bacillus methanolicus NAD-dependent methanol dehydrogenase(mdh) expressed in E.coli

Bacillus methanolicus NAD-dependent methanol dehydrogenase (mdh)

1-CSB-YP330043BAU Cusabio
  • 814.80 EUR
  • 402.00 EUR
  • 2606.40 EUR
  • 1261.20 EUR
  • 1730.40 EUR
  • 522.00 EUR
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Bacillus methanolicus NAD-dependent methanol dehydrogenase(mdh) expressed in Yeast

1000 ug/mL Thiram in LCMS Methanol

LCS-3499 Scientific Laboratory Supplies 1ML 135.66 EUR

Tetraphenylrecorcinol bis(diphenylphosphate) 50ug/mL in Methanol

REPBDE0509 Scientific Laboratory Supplies 1ML 1857.06 EUR

Tris-(aziridinyl)-phosphineoxide 100ug/mL in Methanol

REPBDE0510 Scientific Laboratory Supplies 1ML 511.86 EUR

Tris-(aziridinyl)-phosphineoxide 500ug/mL in Methanol

REPBDE0511 Scientific Laboratory Supplies 1ML 591.66 EUR

Cannabichromene (CBC) 1000 ug/mL in Methanol

S-10248 Scientific Laboratory Supplies 1ML 238.26 EUR

1000 ug/ml beta-Myrcene in Methanol

S-2654-MEOH Scientific Laboratory Supplies 1ML 80.94 EUR

1000 ug/ML Perfluorooctanoic Acid in Methanol

S-4460-MEOH Scientific Laboratory Supplies 1ML 71.82 EUR
×

The outcomes confirmed that SBC was the most enough for the accessible knowledge, including insignificant error to the base mannequin estimates. Single wavelength standardization was a shut second finest, probably extra strong, and impartial of the base iPLS mannequin. Local centering was proven to be insufficient for the samples and devices used.

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Cell biologist studying how a heart grows and dies. Cell Division; Cell Enlargement; Cell Death. Artist and fashion designer. Associate Professor. (he/him)

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Two cells photographed through a microscope. DNA in the nucleus, the endoplasmic reticulum, and actin filaments are shown.
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Three cells photographed through a microscope. DNA in the nuclei (blue), mitochondria (green) and actin filaments (red) are shown. #CellBiology

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A cell going through cell division videoed through a microscope. DNA (red), Golgi (green) and actin (blue) are shown. XZ view (top) and XY view (bottom) #CellBiology

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The dance sequence at the end of Disney’s Descendants 2 is epic.

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